Cellquest is the data acquisition and analysis program from bd bioscience and operates in the macintosh environment on the facscalibur cytometers. Facsdiva software the software uses a database server software to manage the flow cytometry data. If you are a new user, use the tutorials to get started with the software. Carleton stewart and rpci apoptosis prep for training. I only came upon this after our bd service engineer sent me a copy of it. Aug 17, 2017 running a basic 2 color flow cytometry experiment in bd facs diva uc merced scif stem cell instrumentation foundry. The wide range of product courses includes handson, instructorled training, as well as elearning in instructor led virtual classrooms, selfpaced study, and online webinars. Running a basic 2 color flow cytometry experiment in bd facs. Aug 25, 2017 this video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Refer to the information supplied by the manufacturer for applicationspecific limitations.
Running a basic 2 color flow cytometry experiment in bd facs diva. If you are an experienced user, try the updated tutorials to. Apr 03, 2014 here well have a discussion covering many topics relating to the bd facsdiva platform including software and hardware. Firsttime users of bd facsdiva software should read.
I had the same problem before and i hoped somebody would come up with a better solution than mine. Representative ctn data acquired on a bd facsverse system. Developed in collaboration with data innovations, a leading middleware provider experienced in clinical applications, this laboratory information system lis interface solution allows the direct transfer of information between bd software and an lis, reducing errors commonly caused. Upon startup, the software will connect to the cytometer. The format of the user name for facsdiva software is the first letter of your first name followed by your full last name. See headers and footers on page 100 in the bd facsdiva software reference manual. Bd facsdiva software overview selfpaced data display in bd facsdiva software selfpaced using the worksheet window in bd facsdiva software selfpaced using templates in bd facsdiva software selfpaced bd facsvia online training program. Verify that the breakoff is stable and turn on the sweet spot. Feb 25, 20 how to use experiment layout in facsdiva raffaello cimbro. This video describes how to set up an experiment in facs diva version 8. Bd facsdiva software features organization bd facsdiva. If you are not familiar with the windows operating system, see the documentation provided with your computer. The lsrii, fortessa, x20, and symphony appear different, but the operation and workflow are performed in the same manner on all instrument types. Regarding this runtime module, you are prohibited from.
May 17, 20 bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. To log into the computer, use lsrii as the user name and lsrii as the password. Allow the instrument to connect to the software if necessary. This version runs on the windows 7 32bit operating system. The first, oddly enough, is bds very own index sorting manual tutorial. Bd cytometer setup and tracking application guide cytometer configuration setup bd facsdiva.
Bd biosciences digital flow cytometry software provides. Compensation of a 7 color panel on the bd lsr ii uc merced scif stem cell instrumentation foundry. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. A forward scatter vs side scatter of ctn using bd facsuite software b plot showing aggregates on the basis of propidium iodide width w vs propidium iodide area a c resolution and linearity shown by a propidium iodide area histogram of propidium iodide stained ctn. Bd facsdiva software reference manual for version 7. Using application settings to standardize flow cytometry. At the heart of the bd lsr ii is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell. Tips and tricks to help streamline your facsdiva experience. The most popular version of this product among our users is 4. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Running a basic 2 color flow cytometry experiment in bd. Here well have a discussion covering many topics relating to the bd facsdiva platform including software and hardware. This guide contains instructions for using bd facsdiva software version 6.
To help customers take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications. This video will show you how to install bd facsdiva 8. Refilling sheath tank and emptying waste on bd lsr ii flow cytometer. See using the worksheet grid tab on page 186 in the bd facsdiva software reference manual. Bd compbeads plus bd facsdiva software pdf bd facsaria users guide. This guide contains instructions for using bd facsdiva. Analysis software roswell park comprehensive cancer center. Completion of a bd operator course for a digital cytometer using bd facsdiva software v6 or equivalent experience on a bd digital cytometer. The name of the program executable file is bd facsdiva software. If you are not familiar with the windows operating system, refer to the documentation provided with your computer.
Bd facsdiva software resources software bd biosciences. Cellquest acquistion tutorial cellquest analysis tutorial cellquest users guide vendor website facsdiva is bd biosciences next generation data acquisition and analysis program and operates in the pc windows environment on the facsaria, facscanto. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an experienced user. The bd lsr ii is the first and only aircooled, fourlaser benchtop flow cytometer with the ability to acquire up to 18 colors. Index sorting from facsdiva to flowjo flow cytometry news. For information on how to operate and maintain your flow cytometer, refer to the instrument users. Sophie novault bd facs diva software research institut. For the love of physics walter lewin may 16, 2011 duration. To help customers, take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications.
Our selfpaced elearning courses can be used for basic learning or to refresh and reinforce what you learned in class. Worksheets are cause effect, bd lsrii overview workbook, bd cytometric bead array software users guide, getting started with bd facsdiva software, bd facsdiva software reference manual, chapters 1, practice with all s t be sure to state, bd facsdiva tutorial. For information on how to operate and maintain your flow cytometer, see your cytometer manual. Winlist is a flow cytometry analysis software that operates in the pc windows environment. Compensation of a 7 color panel on the bd lsr ii youtube.
Hts option for bd lsr ii 9 7 at the log in dialog, choose your user name, enter your password, and click ok. Facsdiva is bd biosciences next generation data acquisition and analysis program and operates in the pc windows environment on the facsaria, facscanto, lsrii and fortessa. Most software functions are controlled using the tool bar at the top of the workspace and toolbars within the browser and worksheet windows. Using templates in bd facsdiva software selfpaced bd facsvia online training program. For the demonstration, we will assume this is a 4color flow cytometric analysis. Mar 01, 2015 the first, oddly enough, is bd s very own index sorting manual facsdiva software manual and may not even be installed on your computer or available for download from bd s website. Since efficient imagestream data analysis demands ample computer storage space, powerful processing speed, and fast network connections, dedicated 64 bit. Dealing with a clog on the bd lsr ii flow cytometer duration. This manual describes how to use bd facsdiva software. For the demonstration, we will assume this is a 4color flow cytometric analysis experiment. Next you want to tell the software what populations to show in each plot. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an.
This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Download bd facsdiva software by becton, dickinson and company. Bd facsdiva software reference manual for version 8. The runtime module was developed by a third party and we are obligated to notify our customers about the limitations for use. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Turn on the instrument using the green power button. Download bd facsdiva software by becton, dickinson and. In the login window, scroll down through the list of user names to locate yours. Access bd lsr ii online help from the help menu of bd facsdiva software. Expand the specimen and make the tube active by clicking the arrow next to. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password. Bd facsmelody online training program additional content available in the elearning library. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments.
Interference there are no known incompatibilities with bd facscanto clinical. The letter also stated that bd biosciences rep will contact the users when the new software is available. Try free download manager fdm alternative software 1. Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. Bd facsdiva software, free download by becton, dickinson and company. Welcome to the introduction to bd facslink lis interface solution with bd facsdiva software webbased training. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. Bd facsdiva software features bd facsdiva software bd. Dear all, a while ago there was a thread about how to recover deleted bd facsdiva files. Bd biosceinces instrument and application trainings.
Cells were sorted on high purity setting at no more than 5000 eventss using a 70 m nozzle at 55 psi with a 90 hz. Lsr iib and lsriic turn on the fluidics pump and ensure that it is running. Download popular programs, drivers and latest updates easily. Bd facsdiva software contains vxworks as embedded software runtime module. User are asked to contact bd customer support center at 18772328995 if they have any additional questions. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample. These uniquely designed, gmp manufactured beads are excited by all currently supplied lasers and emit in the respective detectors for virtually any fluorescent dye. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Proceed to the website of becton, dickinson and company. Carleton stewart and rpci titering directly conjugated antibodies to extracellular antigens special thanks to dr. When autoplay is enabled, a suggested video will automatically play next. If you are an experienced user, try the updated tutorials to become familiar with new software features. Bd lsr ii facility requirement guide contains specifications for.
For instructions on creating a user name and password, refer to the bd facsdiva software reference manual. Includes training materials, snacks, coffee and lunch. All data, plots, gates, instrument settings, and statistics are saved inside the database. The user cannot specify any path for saving, everything is done automatically in. Bd biosciences will release a version of bd facsdiva software to correct this problem. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently.
After a successful login, the bd facsdiva workspace appears showing the main application windows which include browser, cytometer, inspector, acquistion dashboard and global worksheet. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Dec 30, 2017 this video will show you how to install bd facsdiva 8.
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